Inoculate a nutrient broth with the isolate and incubate at room temperature until growth is obtained, usually 24 hours. After incubation use a sterile loop
https://www.fws.gov/policy/fh_handbook/Volume_2/Chapter 5 - ...

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Note added at 13 mins (2021-07-29 13:51:24 GMT)
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In this technique, a drop of the culture on the end of a thin, sterile loop of wire, sometimes known as an inoculator, is streaked across the surface of the agar leaving organisms behind, a higher number at the beginning of the streak and a lower number at the end.
https://en.wikipedia.org/wiki/Agar_plate

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Tilt the tube to bring the broth culture within 1 inch from the lip of the tube. Insert
the sterile loop and remove a small amount of growth; a loopful is usually
sufficient. Try not to touch the sides of the tube with the loop.
https://www.sas.upenn.edu/LabManuals/biol275/Table_of_Conten...

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Fisherbrand™ Disposable Inoculating Loops and Needles, Flexible Needle and Loop
https://assets.fishersci.com/TFS-Assets/CCG/product-images/C...

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